Georgetown University comprises two unique campuses in the nation’s capital. With the Hilltop Campus located in the heart of the historic Georgetown neighborhood, and the Capitol Campus, just minutes from the U.S. Capitol and U.S. Supreme Court, Georgetown University offers rigorous academic programs, a global perspective, and unparalleled opportunities to engage with Washington, D.C. Our community is a close-knit group of remarkable individuals driven by intellectual inquiry, a commitment to social justice, and a shared dedication to making a difference in the world.
Requirements
As a top academic health and science center in the United States, Georgetown University Medical Center seeks to provide, in a synergistic fashion, excellence in education — training physicians, nurses and other health care professionals, as well as biomedical scientists — and cutting-edge interdisciplinary research collaboration, enhancing our basic science and translational biomedical research capacity in order to improve human health. Emphasizing service, we are dedicated to the Catholic, Jesuit principle of cura personalis — “care of the whole person.” Our partnerships with organizations in the region strengthen our work and expand its reach.
The Research Assistant III (Laboratory Technician) works within the Department of Microbiology and Immunology in a position critical to the successful implementation of grant-funded studies in the woodchuck animal model, and as such, it is expected that the applicant has prior laboratory experience and is familiar with routine assays and methods, such as ELISA and quantitative PCR. Together with the PI, they coordinate and manage woodchuck-specific sample processing and then performs all assays/methods independently, including the determinations of viral titers and immune responses in blood, liver and other tissues of woodchucks. This individual is responsible for the handling and processing of stat and archived samples. They eventually train and supervise Undergraduate and Graduate students during internships in relevant sample processing and laboratory assays. This position also involves shipment of samples to diagnostic or collaborating laboratories. Duties include but are not limited to:
Process woodchuck samples (blood, serum, liver, other tissues).
Oversee quantitative viral assays woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV), including slot blots for viral DNA, Southern and Northern hybridization for viral DNA and RNA, and real time PCR for viral DNA and RNA.
Host immune response assays, including proliferation assays for T-cells, ELISpot assays for IFN-gamma secreting T-cells, FACS-based degranulation assays for cytotoxic T lymphocytes, real time PCR for immune markers (cytokines and leukocyte surface markers) in whole blood lysates and liver biopsies.
Manage ELISAs for viral proteins or antibodies against these proteins.
Organize sample records and data from the above assays in standard spread sheets.
Work Interactions
The Department of Microbiology and Immunology is dedicated to the study of infectious diseases caused by diverse pathogens, including basic research on and treatment of HBV in the woodchuck animal model. The individual is a team member of the PI’s group and critical for the sample processing and analysis from ongoing and future woodchuck studies. He/she reports directly to the PI. They will interact with other members of the PI’s group to coordinate sample processing and analysis. They also interact further with Undergraduate and Graduate students concerning sample processing and analysis.
Requirements and Qualifications
Prior experience in performing sample processing and analysis under supervision and/or independently
Ability to follow establish standardized protocols and procedures for routine use in the laboratory, as applied to woodchuck samples (i.e., most assays are lab-generated and cannot be purchased commercially)
Ability to perform new protocols and procedures like the above assays including applications of new reagents, and to use appropriate methodologies to validate results
Ability to conduct analysis to ensure correct and precise specificity of signals against appropriate standards and other controls
Ability to ensure that high quality results are achieved in a timely and organized manner and that data obtained can meet the scientific objectives of the study
Preferred Requirements and Qualifications
Bachelor’s degree or higher with prior laboratory experience in general for several years (2-5 years)
A preference will be given to individuals with prior experience with viral and host assays for HBV or other viruses
Must be willing and able to acquire skills and experience with woodchuck sample-specific assays - Note that some of the above assays may require university certification for direct handling and conducting assays with radioisotopes (32P)
Work Mode Designation
This position has been designated as On-Campus. Please note that work mode designations are regularly reviewed in order to meet the evolving needs of the University. Such review may necessitate a change to a position’s mode of work designation. Complete details about Georgetown University’s mode of work designations for staff positions can be found on the Department of Human Resources website: https://hr.georgetown.edu/mode-of-work-designation.
Pay Range:
The projected salary or hourly pay range for this position which represents the full range of anticipated compensation is:
$18.28 - $27.33Compensation is determined by a number of factors including, but not limited to, the candidate’s individual qualifications, experience, education, skills, and certifications, as well as the University’s business needs and external factors.
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EEO Statement:
Georgetown University is an Equal Opportunity/Affirmative Action Employer fully dedicated to achieving a diverse faculty and staff. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, national origin, age, sex (including pregnancy, gender identity and expression, and sexual orientation), disability status, protected veteran status, or any other characteristic protected by law.
Benefits:
Georgetown University offers a comprehensive and competitive benefit package that includes medical, dental, vision, disability and life insurance, retirement savings, tuition assistance, work-life balance benefits, employee discounts and an array of voluntary insurance options. You can learn more about benefits and eligibility on the Department of Human Resources website.